The interaction of reduced nicotinamide–adenine dinucleotide phosphate with reduced nicotinamide–adenine dinucleotide–ubiquinone reductase from bovine heart mitochondria

Ubiquinone Reductase from Bovine Heart Mitochondria

Purification and Characterization of a Reduced Nicotinamide Adenine Dinucleotide Phosphate-linked Aldehyde Reductase from Brain*

The presence of a nonspecific NADPH-linked aldehyde reductase (alcohol: NADP oxidoreductase, EC 1.1.1.2) was observed in various areas of bovine brain in vitro. In the midbrain, hypothalamus, and pons, the rate of NADPH oxidation, with p-nitrobenzaldehyde as substrate, was approximately 4.0 nmoles per min per mg of protein; but somewhat lower values were obtained when other regions of the brain...

Metmyoglobin reductase. Identification and purification of a reduced nicotinamide adenine dinucleotide-dependent enzyme from bovine heart which reduces metmyoglobin.

Beef heart muscle has been found to contain an enzyme which will rapidly and directly reduce metmyoglobin in vitro. Reduction rates are far greater than any previously reported for nonspecific or nonenzymatic systems. The enzyme is NADH-dependent and requires the presence of ferrocyanide ion for in vitro assay. The artificial electron carriers, dichlorophenolindophenol and methylene blue, are n...

Reduced nicotinamide-adenine dinucleotide-nitrite reductase from Azotobacter chroococcum.

1. The assimilatory nitrite reductase of the N(2)-fixing bacterium Azotobacter chroococcum was prepared in a soluble form from cells grown aerobically with nitrate as the nitrogen source, and some of its properties have been studied. 2. The enzyme is a FAD-dependent metalloprotein (mol.wt. about 67000), which stoicheiometrically catalyses the direct reduction of nitrite to NH(3) with NADH as th...

The interaction of reduced pyridine-aldehyde adenine dinucleotide with cytochrome b-5 reductase.

The proposed mechanism for the oxidation of reduced pyridine nucleotides by cytochrome bj reductase (1, 2) (EC 1.6.2.2, reduced nicotinamide adenine dinucleotide (NADH)-cytochrome b5 reductase) is based largely on the characteristics of nucleotide interactions with this flavoprotein and the apoenzyme. The formation of a stable complex of NAD, or any one of several analogues of this nucleotide, ...